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1、JournalofMedicalVirology11:3l9-325(L983)DiagnosisofHumanCoronavirusInfectionsinChildrenUsingEnzyme-LinkedImmunosorbentAssayMalcolmR.Macnaughton,DianneFlowers,andDavidlsaacsDivisionofCommunicableDiseases,ClinicalResearchCentre,Harrow,Middlesex,EnglandAnenzyme-linkedimmunosorbentassay(ELISA)wasdev
2、elopedfordiagnosinghumancoronavirus(HCV)infectionsinchildren.Onehundredandseventysevennoseswabs,throatswabs,andnasopharyngealaspirateswerecollectedfrom30childrensufferingfromacuterespiratoryinfections.ThesesamplesweretestedforHCVantigensbyELTSAand28.2%ofthesampleswereshowntobeHCVpositive.Thesere
3、sultsindicatethatourELISAshouldproveusefulinthediagno-sisofHCVinfectionsinchildren.FurtherstudiesareinprogresstoextendtheELISAtodetectHCVsinexperimentallyandnaturallyacquiredinfectionsinadults.Keywords:HCVinfections,HCVdiagnosis,HCVinchildren,ELlSAforHCVsINTRODUCTIONHumancoronaviruses(HCVs)cause
4、mildupperrespiratorytractinfectionsorcommoncoldsinbothadultsandchildren:upto30%ofcoldshavebeenassociatedwithHCVs[Larsonetal,1980;McIntosh,1974;Monto,19741.However,mostpreviousstudieshaveusedchangesinserumantibodiestoconfirmHCVinfections[BradburneandTyrrell,1971;McIntosh,1974;Monto,19741.Fewstudi
5、eshavereportedtheisolationordetectionofHCVsinnasalsecretionsduetothefastidiousgrowthrequirementsofmostHCVisolatesintissueandorganculture[HamreandProcknow,1966;Larsonetal,1980;McIntoshetal,1967;TyrrellandBynoe,19651.Nevertheless,onestudyhasusedimmunofluorescencetodetectHCVantigensinepithelialcell
6、sshedfromthenasopharynxofvolunteersinoculatedwithHCVs[McIntoshetal,19781.However,noHCVsweredetectedbythismethodinsimilarcellsshedinthenasalsecretionsofchildrenwithnaturallyacquiredrespiratoryinfections[McIntoshetal,19781.AcceptedforpublicationOctober26,1982.Addresscorrespondenceto:MalcolmR.Macna
7、ughton.DivisionofCommunicableDiseases,ClinicalResearchCentre,WatfordRoad,Harrow,MiddlesexHA13UJ,England.01983AlanR.Liss,Inc.320Macnaughton,Flowers,andIsaacsInthispaper,wedescribeanELISAtodetectHCVs,frombothHCVantigenicgroups
