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1、996MicroscMicroanal11(Suppl2),2005DOI:10.1017/S1431927605500278Copyright2005MicroscopySocietyofAmericaHostcellchangesinducedduringSevereAcuteRespiratorySyndrome(SARS)coronavirusinfection.12123MLNg,SHTan,JWMLee,MLNLeongandYJTan.1FlavivirologyLaboratory,DepartmentofMicrobiology,5ScienceDrive2,Nationa
2、l2UniversityofSingapore,Singapore117597.ElectronMicroscopyUnit,12MedicalDrive,FacultyofMedicine,NationalUniversityofSingapore,Singapore117597.3InstituteofMolecularandCellBiology,61BiopolisDrive,Proteos,Singapore138673.IntroductionAnewvirusinfectionstartedinsouthernChinainNovember2002andsubsequently
3、spreadtoHongKong,Vietnam,CanadaandSingapore.TheSevereAcuteRespiratorySyndrome(SARS)viruswasidentifiedtobeanewvirusthatbelongstothecoronavirusfamily(Drostenetal.,2003;Ksiazeketal.,2003).Unliketheothermemberswhichcausemildrespiratorysymptoms(Holmes,1990),theSARScoronavirusresultinfatalitiesrangingfro
4、m10to15%oftheinfectedpatientsdependingontheagegroup.Thereplicationcyclewassimilartotheothercoronavirusesinthatthelatentperiodwasabout6hoursincellcultureshowevertheproductionofprogenyviruswasveryefficient(Sturman&Takemoto,1972;Ngetal.,2003).Microscopyinvestigationwasundertakentoseeifanyobservedhostc
5、hangesinducedbythevirusinfectioncouldhelpexplaintheseverityofthedisease.MethodsandMaterialsTheTEM,AFMandSEMwereusedtostudythemorphologicalchangesintheinfectedcells.Immuno-TEMwasalsousedtolocalizeauniqueSARSvirusprotein3a(U274)inrelationtotheinducedmorphologicalchanges.Attemptsweremadetolookforvirus
6、localizationandinducedmorphologicalchangesintheautopsylungtissuesusingtheTEM.ResultsandconclusionsIninvitrostudiestheSARSviruswasfoundtoreplicateatgreatspeedandproducedhighnumbersofprogenyvirusparticleswithin12to15hourspostinfection.Theearlychangesentailtheappearanceofthelargevacuolesenclosinglarge
7、myelin-likemembranewhorls.Thesemembranescouldhavearoleintheearlyeventofreplicationastheyareseencloselyassociatedwiththeuncoatedvirusnucleocapsids.Thesemembranestructuresremainedprominentduringthefirst10hour
