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1、JournalofVirologicalMethodsELSEVIERJournalofVirologicalMethods55(1995)175-183Detectionofhumancoronavirus229E-specificantibodiesusingrecombinantfusionproteinsA.Pohl-Koppe*,T.Raabe,S.G.Siddell,V.terMeulenInstituteofVirologyandImmunology,UniuersityofWiir
2、zburg,VersbacherStr.7,97078Wiirzburg,GermanyAccepted13May1995AbstractHumancoronavirusesareknowntobeacommoncauseofrespiratoryinfectionsinman.However,thediagnosisofhumancoronavirusinfectionsisnotcarriedoutroutinely,primarilybecausetheisolationandpropaga
3、tionofthesevirusesintissuecultureisdifficultandtimeconsuming.Theaimofthisstudywastoevaluatetheuseofrecombinant,bacterialexpressedproteinsintheserodiagnosisofcoronavirusinfections.Twoproteinswereexamined:thehumancoronavirus229Enucleocapsidprotein(N),ex
4、pressedasafusionproteininthevectorpURandthecoronavirus229Esurfaceglycoprotein(S),expressedasafusionproteininthevectorPROS.TherecombinantproteinswereusedasantigensinWesternblot(WB)assaystodetectthe229E-specificIgGantibodiesandtheresultswerecomparedwith
5、astandardserologicalmethod,indirectimmunofluorescence.Serumsamplesof51paediatricpatients,sufferingfromacuterespiratoryillness,and10adults,voluntarilyinfectedwithhumancoronavirus,weretested.Thesemmsamplesoftheadultgrouphadcoronavims-specificIgGantibodi
6、esinbothtestsystems.Incontrast,only8/51seraofthepaediatricgroupwerepositiveforcoronavirus-specificIgGbybothWBandIFand20/51serawerepositivebyWB,butnotbyIF.Theoverallincidenceofhumancoronavimsinfectionsinthepaediatricagegroupwas55%evaluatedbyWBanalysisa
7、nd16%evaluatedbyIF.Thisstudyshowsthatrecombinanthumancoronavirus229Eproteinsaresuitablereagentsfortheepidemiologicalscreeningofcoronavirus229Einfections.Keywords:Coronavirus;229B,Bacterialfusionprotein;Antibodydetection*Correspondingauthor.Presentaddr
8、ess:Dr.vonHaunerschesKinderspital,Lindwurmstr.4,8000Miinchen2,Germany.Presentaddress:InstitutfiirGenetik,Theodor-Boveti-InstitutfiirBiowissenschaften,97074Wdrzburg,Germany.0166-0934/95/$09.5001995ElsevierScienceB.V.AllrightsreservedSSDIO166-09
