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1、JournalofClinicalVirology30(2004)211213Theantiviraleffectofinterferon-betaagainstSARS-CoronavirusisnotmediatedbyMxAproteinMartinSpiegela,AndreasPichlmaira,ElkeMühlbergerb,OttoHallera,FriedemannWebera,?aAbteilungVirologie,InstitutfürMedizinischeMikrobiologieundHygiene,Universit?tFreiburg,D-79008F
2、reiburg,GermanybInstitutfürVirologiederPhilipps-Universit?tMarburg,35037Marburg,GermanyReceivedinrevisedform6November2003;accepted14November2003AbstractSevereacuterespiratorysyndrome(SARS)iscausedbyanovelcoronavirustermedSARS-CoV.Noantiviraltreatmenthasbeenestab-lishedsofar.Interferonsarecytokin
3、eswhichinducethesynthesisofseveralantivirallyactiveproteinsinthecell.Inthisstudy,wedemon-stratedthatmultiplicationofSARS-CoVincellculturecanbestronglyinhibitedbypretreatmentwithinterferon-beta.Interferon-alphaandinterferon-gamma,bycontrast,werelesseffective.ThehumanMxAproteinisoneofthemostpromin
4、entproteinsinducedbyinterferon-beta.Nevertheless,nointerferencewithSARS-CoVreplicationwasobservedinVerocellsstablyexpressingMxA.Therefore,otherinterferon-inducedproteinsmustberesponsibleforthestronginhibitoryeffectofinterferon-betaagainstSARS-CoV.?2003ElsevierB.V.Allrightsreserved.Keywords:SARS-
5、Coronavirus;Interferon;MxAprotein;Virusinhibition1.Introductioninestablishinganantiviralstate.TypeIIFN(IFN-/)issynthesizedbymostcelltypesasadirectresponsetovirusSevereacuterespiratorysyndrome(SARS)isaninfec-infection,whereastypeIIIFN(IFN-)isproducedbyim-tiousdiseasewhichhasrecentlyemergedinCh
6、inaandmunecellsaftercontactwithantigen-presentingcells.Bothrapidlyspreadtoothercountries(Chanetal.,2003).TotypeIandtypeIIIFNsinducethesynthesisofdistinctdate,8098caseswith774deathsarereported(WHO,butpartiallyoverlappingsetsofmRNAswhichencodepro-2003).Intensiveresearchhasledtotheidenti?cationofte
7、inswithantiviral,antiproliferative,andimmunomodula-apositive-strandedRNAvirus,termedSARS-coronavirustoryproperties(deVeeretal.,2001).AmongthetypeI(SARS-CoV),astheetiologicagent(Drostenetal.,2003;IFN-inducedproteins,themainan
