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1、$16JournalofClinicalVirology2006,Vol36(suppl2)Abstracts,9thESCVAnnualMeetingIIdentificationofrespiratoryvirusesdetectedduringthreelP~Diagnosisofhumanmetapneumovirusbyconsecutivewinterseasonsimmunofluorescence-theNewcastleexperienceE.Pagani1,C.Petters1,D.Secolo1,M.Casini2,L.Pescollderungg3
2、,C.Taylor1,Y.Taha1,B.Young1,G.Toms2,F.Fenwick2,B.Moser4,E.Percivalle5,H.RHuemer6,C.Larcher1.1LaboratoryR.McGuckin2.1HealthProtectionAgencyLaboratory,NewcastleofMicrobiologyandVirology,2DepartmentofHaematology,UponTyne;2UniversityofNewcastleUponTyne,NewcastleUpon3DepartmentofPediatrics,4De
3、partmentofIntensiveCareTyne,UKMedicine,BolzanoGeneralHospital,Bolzano,Italy,5ServiziodiVirologia,IRCCSPoliclinicoSanMatteo,Pavia,Italy,DepartmentBackgroundandAims:Humanmetapneumovirus(hMPV)wasofHygiene,MicrobiologyandSocialMedicine,InnsbruckMedicaldiscoveredin2001.hMPVhasaworldwidedistrib
4、utionandhasUniversity,Innsbruck,AustriaaspectrumofrespiratoryillnessinchildrensimilartoRespiratorySyncytialVirus(RSV).TheaimofthestudywastoinvestigateInordertoidentifythedifferentviralagentsmorefrequentlyinvolvedtheprevalenceofhMPVinfectioninhospitalizedpatientsinnorthinmildandsevererespi
5、ratorytractinfections,614symptomaticeastEnglandduringApril2005toApril2006usingimmunofloures-patients,351attendingthepediatricand263thehematologyunitscence(IF).oftheGeneralHospitalofBolzano,Italy,wereinvestigateddur-Methods:ApolyclonalantiserumtohMPVwaspreparedandingthreeconsecutiveseasons
6、,fromOctober2003throughAprilusedinanindirectIFassay.Thereagentwasvalidatedagainst2006.Bronchoalveolarlavage,bronchialaspirate,nasopharyngealaninhousehMPVRTPCR(FandNgenetargets).IFwasalsosecretionandnasopharyngeallavagespecimenswereexaminedperformedusingapoolofmonoclonalantibodiesandcompar
7、edbydirectfluorescentantibodyassayand,wherepossible,shellvialwiththepolyclonalantiserum.culturetechniqueforthepresenceofinfluenzavirusA/B(FluA/B),Results:IndirectIFusingthepolyclonalantiserumhadasensi-Parainfluenzavirus1,2,3(PIV1,2,3),Adenoviruses(Adeno),Res-tivityo
