冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf

冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf

ID:51829051

大?。?80.27 KB

頁數(shù):8頁

時間:2020-03-16

冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf_第1頁
冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf_第2頁
冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf_第3頁
冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf_第4頁
冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf_第5頁
資源描述:

《冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ A Multiplex Polymerase Chain Reaction for Differential Detec.pdf》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫

1、Chapter12AMultiplexPolymeraseChainReactionforDifferentialDetectionofTurkeyCoronavirusfromChickenInfectiousBronchitisVirusandBovineCoronavirusChienChangLoa,ChingChingWu,andTsangLongLinAbstractAmultiplexpolymerasechainreaction(PCR)methodfordifferentialdetectionofturkeycoronavirus(TCoV),infecti

2、ousbronchitisvirus(IBV),andbovinecoronavirus(BCoV)ispresentedinthischapter.Primersaredesignedfromtheconservedorvariableregionsofnucleocapsid(N)orspike(S)proteingenesofTCoV,IBV,andBCoVandusedinthesamePCRreaction.ReversetranscriptionfollowedbyPCRreactionisusedtoamplifyaportionofNorSgeneoftheco

3、rrespondingcoronaviruses.TwoPCRprod-ucts,a356-bpbandcorrespondingtoNgeneanda727-bpbandcorrespondingtoSgene,areobtainedforTCoV.Incontrast,onePCRproductof356bpcorrespondingtoafragmentofNgeneisobtainedforIBVstrainsandonePCRproductof568bpcorrespondingtoafragmentofSgeneisobtainedforBCoV.KeywordsM

4、ultiplexPCR,Differentialdetection,Coronavirus,Nucleocapsidgene,Spikegene1IntroductionTurkeycoronavirus(TCoV)contributedtosigniTcanteconomiclossesandremainsasaseriousthreattotheturkeyproducers.Turkeycoronaviralenteritisinareaswithhighconcentrationsofturkeysonayear-roundbasisisnoteasilyelimina

5、tedandisencounteredfrequentlyinturkeypoults[1].AccurateandrapidmethodfordiagnosisofTCoVinfectionisthekeytoeffectivecontrolofthedisease.TurkeycoronavirusbelongstothefamilyCoronaviridae,whichisagroupofenveloped,positive-strandedRNAvirusesthatinfectawiderangeofmammalianandavianspecies.Themajors

6、tructuralproteinsofcoronavirusincludephosphorylatednucleocapsid(N)protein,peplomericspike(S)glycoprotein,andtransmembraneormembrane(M)glycoprotein.Spikeproteincontributestothedistinctivepeplomersontheviralsurfaceandcontainsneutralizingandgroup-speciTcepitopes.Spikeproteinishighlyvariableamon

7、gLeyiWang(ed.),AnimalCoronaviruses,SpringerProtocolsHandbooks,DOI10.1007/978-1-4939-3414-0_12,?SpringerScience+BusinessMediaNewYork2016131132ChienChangLoaetal.differentcoronaviruseswhileMandNproteinsaremoreconservedamongcoronavirusesbetweendifferen

當(dāng)前文檔最多預(yù)覽五頁,下載文檔查看全文

此文檔下載收益歸作者所有

當(dāng)前文檔最多預(yù)覽五頁,下載文檔查看全文
溫馨提示:
1. 部分包含數(shù)學(xué)公式或PPT動畫的文件,查看預(yù)覽時可能會顯示錯亂或異常,文件下載后無此問題,請放心下載。
2. 本文檔由用戶上傳,版權(quán)歸屬用戶,天天文庫負責(zé)整理代發(fā)布。如果您對本文檔版權(quán)有爭議請及時聯(lián)系客服。
3. 下載前請仔細閱讀文檔內(nèi)容,確認文檔內(nèi)容符合您的需求后進行下載,若出現(xiàn)內(nèi)容與標題不符可向本站投訴處理。
4. 下載文檔時可能由于網(wǎng)絡(luò)波動等原因無法下載或下載錯誤,付費完成后未能成功下載的用戶請聯(lián)系客服處理。