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1、VeterinaryMicrobiology,9(1984)425--434425ElsevierSciencePublishersB.V.,Amsterdam--PrintedinTheNetherlandsCORONAVIRUSINFECTIONOFTHEBOVINERESPIRATORYTRACTM.S.McNULTY,D.G.BRYSON,G.M.ALLANandE.F.LOGANVeterinaryResearchLaboratoriesStorrnont,BelfastBT43SD(NorthernIreland)(Accepted19March1984)ABS
2、TRACTMcNulty,M.S.,Bryson,D.G.,Allan,G.M.andLogan,E.F.,1984.Coronavirusinfectionofthebovinerespiratorytract.Vet.Microbiol.,9:425--434.Twoviruses,morphologicallyresemblingcoronavirusesandantigenicallyindistin-guishablefrombovineentericcoronavirus,wereisolatedinbovinetrachealorganculturesfrom
3、thelungsandtracheaofyoungcalveswithrespiratorydisease.Intranasalandintratrachealinoculationofthesevirusesintoneonatalcalvesresultedinapredominant-lyupperrespiratorytractinfection,whichwasassociatedwiththedevelopmentofmildrespiratorysymptoms.INTRODUCTIONCoronavirusesarewellestablishedasente
4、ricpathogensinyoungcalves(Tzipori,1981).However,thereisonlyonereportoftheisolationofco-ronavirusesfromthebovinerespiratorytract(Thomasetal.,1982).Thispaperdescribestheisolationofcoronavirusesfromthelowerrespiratorytractsofcalveswithrespiratorydiseaseandtheexperimentalinfectionofyoungcalves
5、withtheseisolatesviatherespiratoryroute.MATERIALSANDMETHODSAntiseraAchickenantiserumtobovineentericcoronavirus,abovineantiserumtobovinevirusdiarrhoea/mucosaldisease(BVD)virusandrabbitantiseratobovinerespiratorysyncytialvirusandparainfluenzavirustype3werepreparedandconjugatedwithfluorescein
6、isothiocyanateasdescribedpre-viously(McNultyetal.,1983).Arabbitantiserumtoastrainofbovineentericcoronavirusisolatedinbovinetracheal-organcultureatthislabor,i-torywaspreparedusingthesameinoculationscheduleasforrespiratorysyncytialvirus.Thisantiserumwasusedintheimmunofluorescencestudiesofexp
7、erimentally-infectedcalves.0378-1135/84/$03.00?1984ElsevierSciencePublishersB.V.426ElectronmicroscopyNegativecontrastelectronmicroscopywascarriedoutonculturemediaharvestedfromtracheal-organculturesandonsamplesofnasalmucusasfollows.Cellsinthesamplesweredeposite
