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1、JournalofGeneralVirology(1990),71,1065-1073.PrintedinGreatBritain1065NucleotidesequenceofthegeneencodingthespikeglycoproteinofhumancoronavirusHCV229EThomasRaabe,BarbaraSchelle-PrinzandStuartG.Siddell*InstituteofVirology,UniversityofWiirzburg,VersbacherStrasse7,8700Wi~rzburg,F.R.G.Thegene
2、encodingthespikeglycoproteinofthehumanbronchitisvirus,felineinfectiousperitonitisvirus,coronavirusHCV229Ehasbeenclonedandse-transmissiblegastroenteritisvirusandmurinehepatitisquenced.ThisanalysispredictsanSpolypeptideofvirus,strainJHMispresented.Wehavealsodonea1173aminoacidswithanM,of128
3、600.TheNorthernblotanalysisofviralRNAsinHCV229E-polypeptidehas30potentialN-glycosylationsites.Ainfectedcellsusingsyntheticoligonucleotides.OnthenumberofstructuralfeaturestypicalofcoronavirusSbasisofthisanalysis,andbyanalogytothereplicationproteinscanberecognized,includingasignalsequence,
4、strategyofothercoronaviruses,weareabletoproposeamembraneanchor,heptadrepeatstructuresandaamodelfortheorganizationandexpressionofthecarboxy-terminalcysteinecluster.Adetailed,comput-HCV229Egenome.er-aidedcomparisonwiththeSproteinsofinfectiousIntroductionprocessinvolvestherecognitionofaspec
5、ificsequence,theso-called'regionofhomology',presentatthe3'endofHumancoronaviruses(HCV)areacommoncauseofaleaderRNAandateachintergenictranscriptionalrespiratorydiseaseinmanandithasbeenestimatedthatreinitiationsiteontheantigenomicRNAtemplate(foratheyareresponsibleforupto20~oofcommoncoldsrev
6、iewseeLaietal.,1987).(Hierholzer&Tannock,1988;Isaacsetal.,1983;InthecaseofHCVthisprocessresultsinasetofsix3'Mclntoshetal.,1974).Withafewexceptions,HCVsarecoterminalsubgenomicRNAs(Kamahoraetal.,1989;difficulttopropagateintissueororgancultureandSchreiberetal.,1989).Byanalogytoothercoronavi
7、rusesconsequentlytheirbiologyisrelativelypoorlyunder-the5'uniqueregionineachRNA(i.e.theregionnotstood.Nevertheless,ithasbeenpossibletoestablishthatpresentinthenextsmallestRNA)shouldbetranslatedtherearetwomajorHCVantigenicgroups,representedand,atleastinthecaseoftheRNAsenco
