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1、JournalofMedicalVirology2:341-346(1978)DiagnosisofHumanCoronavirusInfectionbylmmunofluorescence:MethodandApplicationtoRespiratoryDiseaseinHospitalizedChiIdrenKennethMclntosh,JoyceMcQuillin,SylviaE.Reed,andPhillipS.GardnerDepartmentsofPediatricsandn/licrobiology,UniversityofColoradoMe
2、dicalCenter,Denver,Colorado{K.M.);DepartmentofClinicalVirology,UniversityofNewcastle-upon-Tyne,Newcastle-upon-Tyne,England(J.M.,P.S.G.);andCommonColdUnit,HarvardHospital,Salisbury,Wiltshire,EnglandIS.E.R.)Rabbitantiserawerepreparedagainstcoronavirusstrains229EandOC43andusedsuccessful
3、lytodetectviralantigeninepithelialcellsshedfromthenaso-pharynxofsymptomaticvolunteerswhohadreceivedcoronavirusinoculathreetofourdaysbefore.Thesameserologicreagentswereappliedtonaso-pharyngealsecretioncellsobtainedfrom106infantsandchildrenhospi-talizedwithrespiratorytractdiseaseandapp
4、arentlynotinfectedwithcon-ventionalrespiratoryviruses.Nocoronavirusinfectionsweredetectedbythismethod.ItappearsthatcoronavirusOC43or229EinfectionswerenotcommoninchildreninTynesidehospitalsduringtheperiodofstudy.How-ever,fluorescenceisausefulmethodfordetectionofcoronavirusinfectionsin
5、symptomatichumansubjects.Keywords:coronavirusinfection,immunofluorescence,respiratorydiseaseINTRODUCTIONHumancoronaviruseswerefirstrecoveredfromadultswithcoldsintheearly1960s[5,161.Theirculturerequiredunusualmeasures:eitherblindpassageintissuecultureorinoculationofhumanembryonictrach
6、ealorgancultures.Sincethattime,seroepi-demiologicalstudieshaveimplicatedcoronavirusinfectioninapproximately15%ofalladultcolds[121.Volunteersreceivingsuspensionsofcoronavirusdevelopedcoldsdiffer-ingincertainminorrespectsfromthosefollowingrhinovirusinoculation[2].Infantsandchildrenarea
7、lsosubjecttonaturalinfection,butthediseasespectruminthisagegrouphasbeendifficulttodefine.ReceivedforpublicationMarch20,1978.AddressrepTintrequeststoDrKennethMclntosh,DeptofPediatricsandMicrobiology,UnivofColoradoMedicalCenter,Denver,CO80262.0146-6615/78/0204-0341$01.4001978AlanR.Liss
8、,Inc342McInt
