資源描述：
《冠狀病毒Coronavirusl論文-2002 Detection of Feline Coronavirus in Captive Felidae in the USA.pdf》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、520BriefcommunicationsJVetDiagnInvest14:520±522(2002)DetectionoffelinecoronavirusincaptiveFelidaeintheUSAMelissaKennedy,ScottCitino,AmandaHillisMcNabb,AmySerinoMoffatt,KarenGertz,StephenKaniaAbstract.Felinecoronavirus(FCoV)isanimportantpathogenofdomesticandnondomesticFelidae
2、.In-vestigationintotheprevalenceofFCoVinexoticFelidaehasreliedprimarilyonserology.TheusefulnessofgeneticdetectionofFCoVusingreversetranscriptionandnestedpolymerasechainreaction(RT/nPCR)forviralscreeningwasinvestigated.Seventy-?vebiologicsamples,primarilyfeces,fromcaptivefeli
3、dsfrom11institutionsweretestedusingPCR.Serumsamplescollectedfromallbut12oftheseanimalsweretestedforantibodiestotypeIandtypeIIFCoVbyindirectimmunoˉuorescence.Twenty-fouranimalswerepositiveusingRT/nPCRforvirus.Twenty-nineanimalswereseropositivetotypeIand/ortypeIIFCoV.Fromserol
4、ogicdata,infectionwithavirusantigenicallyrelatedtoFCoVtypeIoccurredmostcommonly.Serologydidnotcorrelatewithvirussheddingbecause13animalswereseronegativetoFCoVtypeIandIIbutpositiveusingRT/nPCRforvirus.Conversely,20animalswereseropositivebutnegativeusingRT/nPCRforFCoV.Someofth
5、epop-ulationsinwhichviruswasdetectedhadexperiencedhealthproblems,includingfelineinfectiousperitonitis(FIP),necrotizingcolitis,andmildenteritis.InadditiontoitsroleinFIP,thisvirusmayplayaroleingastro-intestinaldiseasesofinfectedanimals.ThisstudydemonstratesthatFCoVisasigni?can
6、tinfectiousagentofcaptivefelidsbecauseoverhalfoftheanimalstestedwerepositivebyviralgeneticdetection,serology,orboth.Dependenceupononemethodfordetectionofinfectionisunreliable.Infectiousdiseasesarepotentiallydevastatingtowildlifefrom11zoologicinstitutionsintheUSAforFCoVgeneti
7、cconservation.Inparticular,largecarnivorepopulationsaredetection.SpeciesofFelidaetestedincludedcheetah,snowsusceptibletoseriousconsequencesbecausetheyarealreadyleopard,Africanleopard,serval,Bengaltiger,Siberiantiger,threatenedbyrestrictedranges,habitatdestruction,andover-mou
8、ntainlion,Africanlion,lynx,ocelot,jaguar,andbobcat.exploitationofthemselves
