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1、05-01-0409r.qxp8/14/20063:42PMPage1575IsolationandcharacterizationofacoronavirusfrompigeonswithpancreatitisDongH.Qian,MV;GuoJ.Zhu,DVM;LiZ.Wu,BS;GuoX.Hua,DVMObjective—ToidentifyandpartiallycharacterizeaABBREVIATIONScoronaviruslikevirusisolatedfromnaturallyinfectedIBVInfectiousbronchiti
2、sviruspigeons.TCoVTurkeycoronavirusAnimals—50specificpathogen-free(SPF)embry-SPFSpecificpathogenfreeonatedchickeneggs,30WhiteLeghornSPFchickens,HAHemagglutinationand12clinicallynormalpigeons.RTReversetranscriptionProcedures—PancreatictissuespecimensfromSgeneSpikeglycoproteingenesickpi
3、geonswereinoculatedintoSPFembryonat-PEDVPorcineepidemicdiarrheavirusedchickeneggsforviralisolationandinvestigationHCoVHumancoronavirusofmorphologicandhemagglutinatingpropertiesofSARSSevereacuterespiratorysyndrometheisolate,calledPSH050513.Furthermore,viru-TGEVPorcinetransmissiblegastr
4、oenteritisviruslencestudiesinSPFchickensandexperimentalFCoVFelineinfectiousperitonitisviruspigeonswereperformed.Thespike(S)glycoproteinMHVMurinehepatitisvirusgeneofPSH050513wasfurthersequencedandCCoVCaninecoronavirusanalyzed.BCoVBovinecoronavirusResults—PSH050513wasisolatedandidentifi
5、edfromtheexperimentallyinfectedpigeonsbyaroutinemethod,whichwasinaccordancewithKoch’spostu-Coronaviruseshavebeensubdividedinto3majorlates.ThecompleteSprotein(1,167aminoacids)wasantigenicgroupsonthebasisofantigenicdifferencescomparedwithpublishedSproteinsequencesofidentifiedbyserologic
6、analyses,andthesefindingsotheravianandmammaliancoronaviruses.Ahighhavebeensubstantiatedbynucleotidesequenceanaly-degreeofsequenceidentity(79.3%to99.6%)was5ses.GroupsIandIIcontainseveralmammalianvirus-observedbetweentheSproteinsequenceofPSH050513andpublishedsequencesofavianinfec-es,inc
7、ludingthosethataffecthumans,pigs,cows,tiousbronchitisvirus(IBV);onlylimitedidentity(8、itisv
