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1、ArchivesofVirologyArchivesofVirology73,33--43(1982)?bySpringer-Verlag1982CharaeterizationofaCoronavirusIsolatedfromRatswithSialoadenitisByM.MARUandK.SAToShionogil~esearehLaboratories,Shionogi&Co.,Ltd.,Fukushima-ku,Osaka,Japan"~VithIFigureAcceptedMarch30,1982
2、SummaryAcausativeagent,provisionallydesignatedasCARS,wasisolatedfromtheenlargedsubmaxillaryglandofratwhichwascharacterizedasasialoadenitis,usingmouse-derivedBalb/e3T3cloneA31(3T3)cellculture.Theviruscouldbepropagatedin3T3cellculturewhereitproducedmultinuclea
3、tedgiantceilsandformedclearplaques.Itwasidentifiedasamemberofthecoronavirusgroupfromthefollowingresults:RNAcontentwassuggestedbythelackoftheeffectofcytosinearabinoside,theinfectivitywassensitivetolipidsolventsandin-activatedat56°Cfor5minutes,theviralparticle
4、showedtypicalcoronavirusmorphologywhichwasapproximately100nmindiameter.Serologically,althoughCARSandsialodacryoadenitisvirus(SDAV)actuallybelongedtotherat-corona-virusgroup,someantigenicvariationsexistedbetweenthesetwoagentsintheresultsofbothneutralizationan
5、dcomplement-fixationtestsusingmonovalentantisera.Wheninoculatedintranasallyintosusceptiblerats,CARScausedclini-callyandhistologicallyovertsialoadenitisasobservedinthenaturaloutbreak,andretaineditsvirulenceforratsinseveralpassagesofmousebrainandevenwhenceilcu
6、lturewasused,whileratswhichwereinoculatedwithSDAVwereasymptomatie.IntroductionCoronavirusesappeartobeimportantaetiologicalagentsinexperimentalanimals.Thesevirusesareassociatedwithagreatvarietyofdiseases(15,17,19,22).Forinstance,miceinfectedwithmousehepatitis
7、virusdevelophepatitis,ence-phalitis,chronicimmunologicalmediateddiseases(15,21)anddiarrhoea(12),andinrats,rhinitisandtracheitis(5),pneumonitis(5,16),sialodacryoadenitis(13,23)anddisorderofoesterouscycle(20)werecausedbycoronaviruses.Twodifferentkindsofcoronav
8、irushavebeenisolatedfromrats.OneisParker'sratcoronavirus(RCV)whichcauseslethalinterstitialpneumoniainnewbornrats3Arch.Virol.73/10304-8608/82/0073/0033/$02.2034M.MA~[randK.SATO:(16),andtheotheris
