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1、VeterinaryMicrobiology,5(1980)283--290283ElsevierScientificPublishingCompany,Amsterdam--PrintedintheNetherlandsDIFFERENTIATIONOFCANINECORONAVIRUSANDPORCINETRANSMISSIBLEGASTROENTERITISVIRUSBYNEUTRALIZATIONWITHCANINE,PORCINEANDFELINESERAD.J.REYNOLDS,D.J.GARWESandS.LUCEYA.R.C.InstituteforResearchonA
2、nimalDiseases,Compton,Nr.Newbury,BerksRG16ONN(GreatBritain)(Accepted14July1980)ABSTRACTReynolds,D.J.,Garwes,D.J.andLucey,S.,1980.Differentiationofcaninecoronavirusandporcinetransmissiblegastroenteritisvirusbyneutralizationwithcanine,porcineandfelinesera.Vet.Microbiol.,5:283--290.Monospecificantis
3、erawerepreparedinrabbitsagainstcaninecoronavirus(CCV)andtransmissiblegastroenteritisvirusofpigs(TGEV),andin24pigsand3catsagainstTGEValone.NeutrMizingantibodytitreswerehigherfortheimmunizingthantheheterologousvirus,althoughcross-neutralizationusuallywasdetected.ThisconfirmedthatCCVandTGEVaredistin
4、ct,butantigenicallyrelatedcoronaviruses.Inserafrom41dogs,CCV-neutralizingtitreswereonaverage2.7foldhigherthanTGEV-neutralizingtitres,suggestingthatCCVwasthecausalagent.Serafrom29catsincolonieswithfelineinfectiousperitonitis(FIP)andknowntocontainTGEV-neutralizingantibody,werefoundtohavetitres12.3f
5、oldhigheragainstCCV.TheFIPvirus(FIPV)isprobablymorecloselyrelatedtoCCVthanTGEVasjudgedbyantigensinvolvedinvirusneutralization.Antiseratotwoisolatesofbovinecoronavirus,threeisolatesofhaemagglutinatingencephalomyelitisvirus,sevenstrainsofavianinfectiousbronchitisvirusandthe229Estrainofhumancoronavi
6、rusallfailedtoneutralizeCCVandTGEV.ThusCCV,TGEVandprobablyFIPVfallintoag~oupofantigenicallyrelatedagents,separablefromothermembersofthefamilyCoronaviridae,bybothvirusneutralizationandimmuno-fluorescencetests.INTRODUCTIONAcloserelationshipbetweencaninecoronavirus(CCV)andthecoronavirusesofporcinetr
7、ansmissiblegastroenteritis(TGEV)andfelineinfectiousperitonitis(FIPV)hasbeendemonstratedbyimmunofluorescencetests(Pedersenetal.,1978).Virusneutralization(VN)testswithFIPVitselfhavenotyetbeenpossibleowingtothelackofasimp
