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1、VeterinaryMicrobiology,26(1991)227-239227ElsevierSciencePublishersB.V.,AmsterdamInductionoflactogenicimmunitytotransmissiblegastroenteritisvirusofswineusinganattenuatedcoronavirusmutantabletosurviveinthephysicochemicalenvironmentofthedigestivetractJ.M.Aynauda,S.Bernarda,E.Bottrea
2、ua,I.Lantiera,H.SalmonaandPh.VannierbaInstitutNationaldelaRechercheAgronomique,LaboratoiredePathologiePorcine,37380Nouzilly,FrancebCentreNationald'EtudesV~t~rinairesetAlimentaires,StationdePathologiePorcine,22440Ploufragan,France(Accepted6August1990)ABSTRACTAynaud,J.M.,Bernard,S.
3、,Bottreau,E.,Lantier,I.,Salmon,H.andVannier,Ph.,1991.Inductionoflactogenicimmunitytotransmissiblegastroenteritisvirusofswineusinganattenuatedcoronavirusmutantabletosurviveinthephysicochemicalenvironmentofthedigestivetract.Vet.Microbiol.,26:227-239.Atransmissiblegastroenteritis(TG
4、E)coronavirusmutant(188-SG),selectedasattenuatedandresistanttoacidityandproteasesofthedigestivetractofadultpigs,wasusedasvaccine("Nouzillystrain")insowstoprotectsucklingpigletsagainstachallengeexposurecarriedoutwithahighlyvirulentTGEVstrain.Thepregnantsowswereimmunizedonce(42-49d
5、aysbeforefarrowing)ortwice(42-49and7-15daysbeforefarrowing)bytheoral,intramuscularorconjunctivalroutewiththe188-SGstrain.SowsexposedtovirulentTGEVinthefieldandexperimentallyinfectedsows(twooralinoculationsduringpregnancy)wereusedaspositivecontrolsleadingtohighprotection.Theneutra
6、lizingantibodyresponsetovaccinationand/orinfectionwasstudiedinserumandmilk.Noprotectionagainstmortalitywasobservedinthelittersof(1)thenineseronegative,susceptiblesows,withpigletmortalityof65/70,(2)thesevenonceorallyvaccinatedsows,withmortalityof44/54,(3)thesevensowsvaccinatedtwic
7、ebytheconjunctivalroute,withmortalityof55/76.Moderateprotectionwasobservedin(1)theeightsowsvaccinatedintramuscularlytwicewithpigletmortalityof36/90,(2)thesevenorallyandintramuscularlyvaccinatedsowswithpigletmortalityof31/51.Incontrast,improvedprotectionwasobservedin(1)the10sowsva
8、ccinatedtwiceorally,withpigletmortalityo
