資源描述：
《冠狀病毒Coronavirusl論文-1994 Biological and genetic characterization of a hemagglutinating coronavirus isolated from a diarrhoeic child.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、JournalofMedicalVirology44152-161(1994)BiologicalandGeneticCharacterizationofaHemagglutinatingCoronavirusIsolatedFromaDiarrhoeicChildX.M.Zhang,W.Herbst,K.G.Kousoulas,andJ.StorzDepartmentofVeterinaryMicrobiologyandParasitology,SchoolofVeterinaryMedicine,Louisia
2、naStateUniversity,BatonRouge,Louisiana(X.M.Z.,K.G.K.,J.S.);andZnstitutfurHygieneundInfektionskrankheitenderTiere,Justus-LiebigUniversityGiessen,Giessen,FederalRepublicofGermany(W.H.)ThecoronavirusstrainHECV-4408wasisolatedity,antigenicandnucleotidese-fromdiarr
3、heafluidofa6-year-oldchildwithquencecomparisonsacutediarrheaandpropagatedinhumanrectaltumor(HRT-18)cells.Electronmicroscopyre-vealedcoronavirusparticlesinthediarrheafluidINTRODUCTIONsampleandtheinfectedHRT-18cellcultures.Thisviruspossessedhemagglutinatingandac
4、et-Coronavirusesareimportantcausesofgastroenteri-ylesteraseactivitiesandcausedcytopathicef-tisinanimals,buttheirinvolvementinhumanentericfectsinHRT-18cellsbutnotinMDBK,GBKanddiseasesremainsenigmaticwithevidencelimitedtoFEcells.OneoffourS-specificmonoclonalanti
5、-electronmicroscopicdetectionofcoronavirus-likepar-bodiesreactedinWesternblotswithHECV-4408,ticlesinstoolsamplesobtainedfrompatientswithBCV-L9andBCV-LY138butnotwithHCV-OC43,acutegastroenteritisornecrotizingenterocolitis.At-andtworeactedwithBCV-L9butnotwithHECV
6、-temptstopropagatethesevirusesincellculturesre-4408,BCV-LY138andHCV-OC43.OneS-specificmainedfutilewhileimmunologicalstudiesindicatedandtwoN-specificmonoclonalantibodiesre-thattheyarecoronaviruses[Kiddetal.,1989;Marshallactedwithallofthesestrains.cDNAencompass-
7、etal.,1989;Singhetal.,1989;Battagliaetal.,1987;ingthe3'8.5kboftheviralRNAgenomewasHuang,1987;Schnagletal.,1987;Gernaetal.,1985;isolatedbyreversetranscriptionfollowedbyMortensenetal.,1985;Sitbon,1985;MacNaughtonpolymerasechainreactionamplificationhadsizeandDavi
8、es,1981;Mathanetal.,19751.AnepidemicofandrestrictionendonucleasepatternssimilartonecrotizingenterocolitisinchildrenofaspecialcarethoseofBCV-L9andBCV-LY138.Incontrast,thenurseryinah
