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1、JournalofGeneralVirology(1990),71,2599-2607.PrintedinGreatBritain2599PorcinerespiratorycoronavirusdiffersfromtransmissiblegastroenteritisvirusbyafewgenomicdeletionsDenisRasschaert,MarielaDuarteandHubertLaude*LaboratoiredeVirologieetd'lmmunologieMolOculaires,Institu
2、tNationaldelaRechercheAgronomique,CentredeRecherchesdeJouy-en-Josas,DomainedeVilvert,78350Jouy-en-Josas,FranceThegenomeorganizationofporcinerespiratorycoron-NS3-1,NS4andNS7.PairwisealignmentofPRCVavirus(PRCV),anewlyrecognizedagentwhichhasanucleotideandaminoacidsequ
3、enceswithsequencecloseantigenicrelationshiptotheenteropathogenicdataavailableforthreeTGEVstrainsrevealeda96%transmissiblegastroenteritisvirus(TGEV),wasoverallhomology.However,thegenomeofPRCVstudied.GenomicRNAfromcell-culturedPRCVexhibitedtwoimportantdistinctivefeat
4、ures.Thefirst(FrenchisolateRM4)wasusedtoproduceeDNAwasthattheSgenelacked672nucleotidesinthe5'clonescoveringthegenomic3'endtothestartoftheregionandencodedatruncatedformoftheSspike(S)glycoproteingene(7519nucleotides).Sixpolypeptide,andsecondly,thefirstNSORFdown-openr
5、eadingframes(ORFs)wereidentifiedthatstreamoftheSgenewaspredictedtobenon-functionalallowedthetranslationofthreecoronavirusstructuralasaconsequenceofadoubledeletion.Thesignificanceproteinsandthreeputativenon-structural(NS)poly-ofgenomicdeletionswithrespecttotissuetro
6、pismandpeptides,homologoustoTGEVORFsdesignatedevolutionofcoronavirusesisdiscussed.Introductional.,1988);neutalization-mediatingepitopesuniquetoTGEVhavebeenidentifiedalso(Laudeetal.,1988).Anewporcinerespiratorycoronavirus,tentativelyConversely,anti-PRCVS-specificMAb
7、shavebeendesignatedPRCV,whichhasacloseantigenicrelation-showntodifferentiatebetweenthetwoviruses(O.shiptotheenteropathogenictransmissiblegastroenteri-Deriabine,R.L'Haridon,J.Gelfi&H.Laude,tisvirus(TGEV),suddenlyemergedin1983to1984andunpublisheddata).Examinationofth
8、estructuralpoly-spreadwithinlessthan2yearsinmostifnotallEuropeanpeptidepatternofPRCVvirions(TLM83strain)showncountries,whereisnowpersistsenzootic
